Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Inhibition of LPS-induced TNF- α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and TNF- α analyzed by ELISA. Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Dose-effect relationship between modified citrus pectin (MCP) and honokiol (HNK).

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Modification, Inhibition, Antioxidant Activity Assay, Activity Assay

Journal: bioRxiv

Article Title: Vascular endothelial growth factor receptors 1 and 3 mediate placental trophoblast leptin production in preeclampsia, inducing vascular dysfunction

doi: 10.1101/2025.05.27.656489

Figure Lengend Snippet: Schematic showing immunostaining experiment design ( A) ; immunohistochemistry for BeWo cells treated with a vehicle (top row) or sFlt-1 (bottom row) for 24 hours ( B ); Leptin secretion in media after treatment with vehicle, sFlt-1 (72 hours), VEGF (24 hours), PlGF (24 hours) or combination in Bewo cells (C) ; placental explants from preeclampsia pregnancies (D) or placental explants from normal pregnancies (E); One Way ANOVA, Fisher’s LSD post hoc test, Student’s t-test. *p<0.05, ***p<0.001.

Article Snippet: PLGF, VEGF and Leptin were measured in culture media according to the manufacturer’s protocols (PLGF: #DPG00, R&D Systems; VEGF: #DVR100, R&D Systems; Leptin: RAB0333-1KT, Millipore Sigma).

Techniques: Immunostaining, Immunohistochemistry

Journal: Scientific Reports

Article Title: Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

doi: 10.1038/s41598-017-04669-7

Figure Lengend Snippet: Box plots showed the levels of sCD14 and sCD163 in the serum of controls and excessive drinkers (ED)

Article Snippet: Commercially available enzyme-linked immunosorbent assays were used according to the manufacturers’ protocols for measuring sCD14 (R&D System, Cat#DC140, Minneapolis, MN), and sCD163 (MyBioSource, Cat # MBS728094, San Diego, CA).

Techniques:

Journal: Scientific Reports

Article Title: Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

doi: 10.1038/s41598-017-04669-7

Figure Lengend Snippet: ( A , B ) Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls ( A and B ) and pre-treated with ethanol ranging from 6.25 mM–50 mM (1.4 mg–10 mg%) followed by treatment with LPS (10 ng/ml for 6 hrs). Ethanol primes PBMCs for LPS-induced inflammatory responses, as indicated by the increase in the levels of sCD14 and sCD163 in the supernatant in the dose dependent manner. ( C , D ) PBMCs were isolated from excessive drinkers and subjected to the stimulation with LPS (10 ng/ml for 6 hrs). sCD14 and sCD163 were significantly increased in supernatants of LPS stimulated PMBCs from subjects with EAU compared to controls. *Compared to controls and ^compared to LPS treatment alone, p < 0.05.

Article Snippet: Commercially available enzyme-linked immunosorbent assays were used according to the manufacturers’ protocols for measuring sCD14 (R&D System, Cat#DC140, Minneapolis, MN), and sCD163 (MyBioSource, Cat # MBS728094, San Diego, CA).

Techniques: Isolation

Journal: Scientific Reports

Article Title: Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

doi: 10.1038/s41598-017-04669-7

Figure Lengend Snippet: Relationship between serum LPS and sCD14 ( A ) and sCD163 ( B ). The relationship between serum LPS and sCD14/sCD163 was not linear; however, the correlation was evident when the serum LPS > 2 EU/ml which is consistent with the range we observed subjects with excessive drinking.

Article Snippet: Commercially available enzyme-linked immunosorbent assays were used according to the manufacturers’ protocols for measuring sCD14 (R&D System, Cat#DC140, Minneapolis, MN), and sCD163 (MyBioSource, Cat # MBS728094, San Diego, CA).

Techniques:

Journal: Scientific Reports

Article Title: Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

doi: 10.1038/s41598-017-04669-7

Figure Lengend Snippet: Relationship between serum LPS ( A ), sCD14 ( B ), and sCD163 ( C ) and quantity of alcohol consumption during the last 30 days as measured by timeline follow back. The serum levels of LPS, sCD14, and sCD163 were positively correlated with the quantity of alcohol consumption.

Article Snippet: Commercially available enzyme-linked immunosorbent assays were used according to the manufacturers’ protocols for measuring sCD14 (R&D System, Cat#DC140, Minneapolis, MN), and sCD163 (MyBioSource, Cat # MBS728094, San Diego, CA).

Techniques:

Journal: Scientific Reports

Article Title: Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

doi: 10.1038/s41598-017-04669-7

Figure Lengend Snippet: Serum levels of LPS, sCD14, and sCD163 in response to alcohol cessation. Abstinence was associated with significant decline in the levels of LPS ( A ), sCD14 ( B ) and sCd163 ( C ).

Article Snippet: Commercially available enzyme-linked immunosorbent assays were used according to the manufacturers’ protocols for measuring sCD14 (R&D System, Cat#DC140, Minneapolis, MN), and sCD163 (MyBioSource, Cat # MBS728094, San Diego, CA).

Techniques:

Journal: European journal of immunology

Article Title: Monocyte chemoattractant protein 1 (MCP-1/CCL2) contributes to thymus atrophy in acute myeloid leukemia.

doi: 10.1002/eji.201444736

Figure Lengend Snippet: Figure 2. The MCP-1/CCL2 protein is highly expressed in leukemic mice. C57BL/6 mice were i.p. injected with PBS or the C1498 AML cell line. (A) Cytokine/chemokine expression profiles were analyzed by proteome profiler arrays on thymic tissues 18–24 days postinjection. The four dots in the top row (left and right) and the two dots in the bottom row (left) indicate the positive controls provided by the manufacturer. The numbers indicate (1) MCP-1/CCL2, (2) IFN-γ, (3) IL-1α, and (4) CCL4 pro- teins. Results are shown from a single mouse/condition and are representative of three independent thymi. (B) ZsGreen+ leukemic cell infiltration in the thymus was analyzed by flow cytometry 14, 18, and 24 days after C1498 cells injection. Data are shown as mean ± SD of n = 9–11 mice/time point, and are pooled from four inde- pendent experiments. (C) CCL2 chemokine production in the sera of leukemic mice at different time points was quantified by ELISA. Data are shown as mean ± SD of n = 4–6 mice/time point and are pooled from four inde- pendent experiments. (D) CCL2 chemokine production was quantified by ELISA (left panel) in the supernatants of WT and CCL2-disrupted C1498 cells cultured ex vivo for 18 h and (right panel) in the sera of WT mice 18–20 days after the injection of the WT or the CCL2- nonproducing C1498 cells. Data are shown as mean + SD of n = 3 mice/condition and are representa- tive of two independent experiments. (E) CCL2 mRNA quantification was evaluated by RT-qPCR in leukemic cells ex vivo, in whole and CD4/CD8-depleted thymi from control mice and in thymi from leukemic mice 18–24 days postinjection of WT or CCL2−/−C1498 cells. Data are shown as mean + SD of n = 3 sam- ples/condition, and are representative of two inde- pendent experiments. Thymocytes were isolated from PBS- or C1498-administered mice 18 days postinjection. (F) Surface expression of the known CCL2 chemokine receptors was assessed after staining with the anti- CCR2, anti-CCR4, anti-CCR5 and analyzed by flow cytometry. Data are expressed as mean + SD of n = 8–12 mice/group and are pooled from two indepen- dent experiments. ***p = 0.0007, unpaired Student t-test, comparing C1498- and PBS-injected mice.

Article Snippet: Supernatants from activated splenocytes cultures were quantified for IFN-γ production following the provided protocol of a mouse IFN-γ ELISA kit (R&D Systems).

Techniques: Injection, Expressing, Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture, Ex Vivo, Quantitative RT-PCR, Control, Isolation, Staining